Microscopy core facility
The purpose of the microscopy core facility is to ensure that all employees and postgraduate students of TalTech Department of Chemistry and Biotechnology (DCB) and DCB partners have the opportunity to use DCB light microscopes in their scientific work. The microscopy core facility does not provide imaging service or trainings but helps with smaller problems and coordinates the work in microscopy rooms.
The following microscopes are currently available to researchers:
|
Microscope |
Description |
Price (€/h)* |
|
Olympus BX61 |
upright microscope, brightfield, phase contrast, fluorescence (DAPI, GFP long pass and band pass, Green Ex, Triple), objectives PlanApo 1,25x (NA 0.04), UPlanSApo 4x (NA 0.16), UPlanSApo 10x (NA 0.40), UPlanSApo 20x (NA 0.75), UPlanSApo 40x (NA 0.9), PlanApo N 60x oil (NA 1.42), UPLXAPO 100x oil (NA 1.45), camera Olympus DP70, software Olympus cellSens Standard 1.14 |
4 |
|
Zeiss LSM 900 |
confocal, diode lasers 405, 488, 561 and 640 nm, LED light source Colibri 7, objectives Plan-Apochromat 10x (NA 0.45), Plan-Apochromat 20x (NA 0.8), Plan-Apochromat 63x Oil DIC (NA 1.4), C-Apochromat 63x W Korr UV-VIS-IR (NA 1.2), software ZEN 3.3 (blue edition) |
10 |
|
TIRF (currently not available) |
inverted microscope, fluorescence, laser TIRF (491 & 561 nm), photobleaching, FRAP, CO2 incubator, EMCCD camera iXon 888 (Andor), software Live Acquisition (TILL Photonics) |
7.5 |
|
Zeiss LSM DUO |
confocal, point scanning lasers 405, Ar 458-488-514, 561, 633 nm; line scanning (LIVE scanner) lasers 405, 489, 532; DIC (PMT for bright field), CO2 inkubator, termoadapters, objectives Plan-Apochromat 10x (NA 0.45), Plan-Apochromat 20x (NA 0.8), LCI Plan-Neofluar 63x Korr water/glycerol (NA 1.3), Plan-Apochromat 100x Oil (NA 1.4), software ZEN 2009 |
8.5 |
|
Zeiss Discovery V8 |
stereo microscope, upright and downright lighting, fluorescence (GFP longpass & bandpass), objective PlanApo S 1.0x FWD 60 mm, magnification range 1-8x, camera AxioCam MRc, software ZEN 2 lite. |
2.5 |
|
Zeiss Axiovert |
inverted microscope, adapters for 30-60 mm Petri dishes and 24-96-well plates, phase contrast, PlasDIC, fluorescence (filters 365, 488, 560 nm), objectives Plan-Neofluar 10x (NA 0.3), EC Plan-Neofluar 20x (NA 0.5), LD Plan-Neofluar 40x Korr (NA 0.6), A-Plan 10x Ph1 (NA 0.25), LD A-Plan 20x Ph1 (NA 0.30), LD A-Plan 40x Ph2 (NA 0.50), camera AxioCam MRc5, software AxioVision Rel. 4.8. |
4 |
|
Zeiss SEM |
scanning electron microscope, magnification range <5-106x, pressure 10-3000 Pa, maximum specimen height 145 mm, software SmartSEM, suitable for the imaging of insects and plants |
4 |
|
* Price applies for DCB reasearch groups and students; for other users, 50% of service price and VAT (20%) is added. Invoices are submitted by TalTech DCB once a quarter. |
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Book microscope usage here:
https://outlook.office365.com/owa/calendar/TalTechKBIMikroskoobid@taltech.ee/bookings/
Additional information:
Laura Tamberg
Manager of microscopy core facility
Akadeemia road 15, 12618, Tallinn, room SCI-243
Phone: +372 53478389
E-mail: laura.tamberg@taltech.ee